Abstract
Demand has never been greater for revolutionary technologies that deliver fast, inexpensive and accurate genome information. This challenge has catalysed the development of next-generation sequencing (NGS) technologies. The inexpensive production of large volumes of sequence data is the primary advantage over conventional methods. Here, I present a technical review of template preparation, sequencing and imaging, genome alignment and assembly approaches, and recent advances in current and near-term commercially available NGS instruments. I also outline the broad range of applications for NGS technologies, in addition to providing guidelines for platform selection to address biological questions of interest.
Keywords
DNA sequencingBiologyComputational biologyEmerging technologiesData scienceSelection (genetic algorithm)Computer scienceGeneticsDNAArtificial intelligence
MeSH Terms
AutomationGenetic TechniquesGenomeGenomeHumanGenomicsHumansModelsGeneticMolecular BiologyOligonucleotidesPolymerase Chain ReactionSequence AnalysisDNA
Affiliated Institutions
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Publication Info
- Year
- 2009
- Type
- review
- Volume
- 11
- Issue
- 1
- Pages
- 31-46
- Citations
- 6899
- Access
- Closed
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Cite This
Michael L. Metzker
(2009).
Sequencing technologies — the next generation.
Nature Reviews Genetics
, 11
(1)
, 31-46.
https://doi.org/10.1038/nrg2626
Identifiers
- DOI
- 10.1038/nrg2626
- PMID
- 19997069