Abstract
A highly selective, colorimetric polynucleotide detection method based on mercaptoalkyloligonucleotide-modified gold nanoparticle probes is reported. Introduction of a single-stranded target oligonucleotide (30 bases) into a solution containing the appropriate probes resulted in the formation of a polymeric network of nanoparticles with a concomitant red-to-pinkish/purple color change. Hybridization was facilitated by freezing and thawing of the solutions, and the denaturation of these hybrid materials showed transition temperatures over a narrow range that allowed differentiation of a variety of imperfect targets. Transfer of the hybridization mixture to a reverse-phase silica plate resulted in a blue color upon drying that could be detected visually. The unoptimized system can detect about 10 femtomoles of an oligonucleotide.
Keywords
PolynucleotideOligonucleotideColloidal goldNanoparticleChemistryDenaturation (fissile materials)ColorimetryMaterials scienceNanotechnologyChromatographyDNANuclear chemistryBiochemistry
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Publication Info
- Year
- 1997
- Type
- article
- Volume
- 277
- Issue
- 5329
- Pages
- 1078-1081
- Citations
- 4348
- Access
- Closed
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Cite This
Robert Elghanian,
James J. Storhoff,
Robert C. Mucic
et al.
(1997).
Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles.
Science
, 277
(5329)
, 1078-1081.
https://doi.org/10.1126/science.277.5329.1078
Identifiers
- DOI
- 10.1126/science.277.5329.1078