Abstract
Normal cells in culture invariably undergo senescence, whereby they cease proliferation after a finite number of doublings. Irreversible changes in gene expression occurred in senescent human fetal lung fibroblasts: a non-cell cycle-regulated mRNA was partially repressed; an unusual polyadenylated histone mRNA was expressed; although serum induced c-H- ras , c- myc , and ornithine decarboxylase mRNA normally, ornithine decarboxylase activity was deficient; and serum did not induce mRNA for a replication-dependent histone and for the c- fos proto-oncogene. The loss of c- fos inducibility was the result of a specific, transcriptional block. The results suggest that senescent fibroblasts were unable to proliferate because of, at least in part, selective repression of c- fos ; moreover, the multiple changes in gene expression support the view that cellular senescence is a process of terminal differentiation.
Keywords
Ornithine decarboxylaseBiologyPsychological repressionTranscription (linguistics)Gene expressionMolecular biologyMessenger RNAHistonePolyadenylationCell biologyOrnithine decarboxylase antizymeGeneGeneticsEnzymeBiochemistry
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Publication Info
- Year
- 1990
- Type
- article
- Volume
- 247
- Issue
- 4939
- Pages
- 205-209
- Citations
- 477
- Access
- Closed
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Cite This
Tara Seshadri,
Judith Campisi
(1990).
Repression of c- <i>fos</i> Transcription and an Altered Genetic Program in Senescent Human Fibroblasts.
Science
, 247
(4939)
, 205-209.
https://doi.org/10.1126/science.2104680
Identifiers
- DOI
- 10.1126/science.2104680