Abstract

Isolated hepatocytes, harvested from normal rat livers by portal vein collagenase perfusion, can be attached to collagen-coated dextran microcarriers and transplanted by intraperitoneal injection into rats. Survival and function of the transplanted hepatocytes have been demonstrated in mutant rats lacking bilirubin-uridine diphosphate glucuronosyltransferase activity (Gunn strain) and rats with inherited lack of plasma albumin (Nagase analbuminemia rat strain). This simple technique promises to be useful in the treatment of acute liver failure in humans.

Keywords

MicrocarrierCollagenaseTransplantationAlbuminBilirubinHepatocytePerfusionChemistryIntraperitoneal injectionInternal medicineEndocrinologyLiver functionPortal veinLiver transplantationAndrologyBiologyBiochemistryMedicineIn vitroEnzymeCell

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Publication Info

Year
1986
Type
article
Volume
233
Issue
4769
Pages
1190-1192
Citations
303
Access
Closed

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Achilles A. Demetriou, James F. Whiting, David Feldman et al. (1986). Replacement of Liver Function in Rats by Transplantation of Microcarrier-Attached Hepatocytes. Science , 233 (4769) , 1190-1192. https://doi.org/10.1126/science.2426782

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DOI
10.1126/science.2426782