Abstract
An inhibitor has been identified in the conditioned medium of hamster cells and hamster-human hybrids that suppresses neovascularization in vivo in the rat cornea. Inhibitory activity was tightly linked to the presence of an active cancer suppressor gene in transformants and revertants, in segregating hybrids, and in temperature-limited transformants. It copurified with a approximately 140 kd glycoprotein. Polyclonal antiserum raised against the purified preparation recognized a 140 kd protein in conditioned medium and was able to adsorb out all antiangiogenic activity. These results define the control of the activity of an inhibitor of neovascularization as one function of the cancer suppressor gene active in BHK21/cl13 cells and simultaneously identify a new inhibitor of angiogenesis, a process vital to the growth of solid tumors.
Keywords
BiologyHamsterAngiogenesisNeovascularizationCancer researchTumor suppressor geneCancer cellSuppressorIn vivoMolecular biologyCell biologyCancerGeneBiochemistryCarcinogenesisGenetics
MeSH Terms
Angiogenesis Inducing AgentsAngiogenesis InhibitorsAnimalsCell FusionCell LineCell MovementCell TransformationNeoplasticCorneaEndotheliumVascularGene Expression RegulationGrowth InhibitorsMaleNeoplasmsNeovascularizationPathologicProteinsRatsRatsInbred F344SuppressionGenetic
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Publication Info
- Year
- 1989
- Type
- article
- Volume
- 56
- Issue
- 3
- Pages
- 345-355
- Citations
- 470
- Access
- Closed
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Cite This
Farzan Rastinejad,
Peter J. Polverini,
Noël Bouck
(1989).
Regulation of the activity of a new inhibitor of angiogenesis by a cancer suppressor gene.
Cell
, 56
(3)
, 345-355.
https://doi.org/10.1016/0092-8674(89)90238-9
Identifiers
- DOI
- 10.1016/0092-8674(89)90238-9
- PMID
- 2464438