Recombinant mouse gamma interferon induces the priming step in macrophage activation for tumor cell killing.

J L Pace; Stephen W. Russell; Barbara A. Torres; Howard M. Johnson; Patrick W. Gray

doi:10.4049/jimmunol.130.5.2011

Abstract

Abstract Mouse macrophages become activated to kill tumor cells by traversing a series of steps (1-3). The first of these does not cause the expression of cytolytic activity; instead, it primes macrophages to respond to a second signal(s) that then triggers the onset of killing (4-7). The mediator that is responsible for priming is contained, along with other lymphokines, in the culture supernatants of concanavalin A-stimulated spleen cells (5-7) cloned T lymphocytes (8), or some T cell hybridomas (9). Close association has been noted between macrophage priming activity and antiviral activity that is attributable to gamma interferon (10-12). However, unequivocal evidence that the two activities are products of the same molecule has not been available. We now how conclusively by using mouse gamma interferon (MulFN-gamma)3 produced by recombinant DNA technology that, in addition to antiviral activity, this lymphokine has the capacity to induce the priming step in the process of macrophage activation for tumor cell killing.

Keywords

LymphokinePriming (agriculture)Concanavalin ABiologyInterferon gammaMacrophageCytolysisMolecular biologyImmunologyCytokineImmune systemIn vitroCytotoxicityBiochemistry

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Publication Info

Year: 1983
Type: article
Volume: 130
Issue: 5
Pages: 2011-2013
Citations: 442
Access: Closed

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APA Style

                            
                                
                                    J L Pace, 
                                
                                    Stephen W. Russell, 
                                
                                    Barbara A. Torres
                                
                                et al.
                            
                            (1983). 
                            Recombinant mouse <i>gamma</i> interferon induces the priming step in macrophage activation for tumor cell killing.. 
                            The Journal of Immunology
                            , 130
                            (5)
                            , 2011-2013.
                            https://doi.org/10.4049/jimmunol.130.5.2011
                        

Identifiers

DOI: 10.4049/jimmunol.130.5.2011