Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray

Abstract

A high-capacity system was developed to monitor the expression of many genes in parallel. Microarrays prepared by high-speed robotic printing of complementary DNAs on glass were used for quantitative expression measurements of the corresponding genes. Because of the small format and high density of the arrays, hybridization volumes of 2 microliters could be used that enabled detection of rare transcripts in probe mixtures derived from 2 micrograms of total cellular messenger RNA. Differential expression measurements of 45 Arabidopsis genes were made by means of simultaneous, two-color fluorescence hybridization.

Keywords

DNA microarrayGeneGene expressionMicroarrayDNABiologyMessenger RNAMolecular biologyArabidopsisNucleic acid thermodynamicsRNAComputational biologyGenetics

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Publication Info

Year: 1995
Type: article
Volume: 270
Issue: 5235
Pages: 467-470
Citations: 9537
Access: Closed

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APA Style

                            
                                
                                    Mark Schena, 
                                
                                    Dari Shalon, 
                                
                                    Ronald W. Davis
                                
                                et al.
                            
                            (1995). 
                            Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray. 
                            Science
                            , 270
                            (5235)
                            , 467-470.
                            https://doi.org/10.1126/science.270.5235.467
                        

Identifiers

DOI: 10.1126/science.270.5235.467