Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion.

Michael L. Dustin; Martin E. Sanders; Stephen Shaw; Timothy A. Springer

doi:10.1084/jem.165.3.677

Abstract

CD2 is a T lymphocyte glycoprotein that functions in adhesion of T lymphocytes and also as a putative receptor for activation signals. Functional data suggest that LFA-3, a widely distributed cell surface glycoprotein, may be the biological ligand of CD2. We have purified LFA-3 from human erythrocytes and characterized the purified protein functionally. LFA-3 bound specifically to CD2+ cells, and this binding was inhibited by CD2 mAb. Conversely, purified LFA-3 inhibited binding of CD2 mAb to cells, and the concentration required for this effect suggests that LFA-3 half-saturated CD2 at 1-5 nM LFA-3. Purified LFA-3 inhibited rosetting of human and sheep erythrocytes with CD2+ T lymphoma cells and T lymphocytes, and mediated aggregation of a CD2+ T lymphoma cell line. Purified LFA-3 reconstituted into planar membranes mediated efficient CD2-dependent adhesion of T lymphoblasts. These data demonstrate that LFA-3 is a ligand for CD2 and that LFA-3 can mediate T lymphocyte adhesion.

Keywords

Lymphocyte function-associated antigen 1LymphoblastBiologyCell adhesionMolecular biologyLymphocyteT lymphocyteGlycoproteinAntigenMonoclonal antibodyT cellCell cultureCell biologyAntibodyImmunologyBiochemistryCellImmune system

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Publication Info

Year: 1987
Type: article
Volume: 165
Issue: 3
Pages: 677-692
Citations: 255
Access: Closed

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APA Style

                            
                                
                                    Michael L. Dustin, 
                                
                                    Martin E. Sanders, 
                                
                                    Stephen Shaw
                                
                                et al.
                            
                            (1987). 
                            Purified lymphocyte function-associated antigen 3 binds to CD2 and mediates T lymphocyte adhesion.. 
                            The Journal of Experimental Medicine
                            , 165
                            (3)
                            , 677-692.
                            https://doi.org/10.1084/jem.165.3.677
                        

Identifiers

DOI: 10.1084/jem.165.3.677