Abstract
Kinins in the ascitic fluid from a patient with gastric cancer were purified by gel filtration and reversed-phase high-performance liquid chromatography (HPLC). Two fractions (fractions I and II) showed kinin activity. Fraction I did not correspond to either bradykinin or other known kinins, whereas fraction II corresponded to bradykinin. Fraction I contained 8 amino acid residues from bradykinin minus 1 proline plus 1 additional hydroxyproline. Sequence analysis of fraction I showed that the proline at the third amino acid residue of bradykinin was replaced by hydroxyproline. The retention time of fraction I on reversed-phase HPLC was exactly the same as that of synthetic [hydroxyprolyl3]bradykinin (Arg-Pro-Hyp-Gly-Phe-Ser-Pro-Phe-Arg) and was distinguishable from des-Pro3-bradykinin. Thus, these results demonstrate for the first time the presence of [hydroxyprolyl3]bradykinin in vivo. This is also the first report of the presence of bradykinin in human tumor ascites.
Keywords
BradykininAscitic fluidCancerAscitesIdentification (biology)MedicineChemistryInternal medicineBiology
MeSH Terms
Amino Acid SequenceAmino AcidsAscitesBradykininChromatographyGelChromatographyHigh Pressure LiquidHumansKininsMolecular Sequence DataStomach Neoplasms
Affiliated Institutions
Related Publications
Involvement of the Kinin‐generating Cascade in Enhanced Vascular Permeability in Tumor Tissue
Enhanced vascular permeability in tumor tissue has profound pathological consequences in tumor biology. However, details of the mechanism involved are not clear. The present wor...
Relative reactivity of lysine and other peptide-bound amino acids to oxidation by hypochlorite
Antibacterial and inflammatory responses of neutrophils and macrophages produce hypochlorite as a major oxidant. Numerous side chains of amino acids found in extracellular prote...
Hydrolysis of Biological Peptides by Human Angiotensin-converting Enzyme-related Carboxypeptidase
Human angiotensin-converting enzyme-related carboxypeptidase (ACE2) is a zinc metalloprotease whose closest homolog is angiotensin I-converting enzyme. To begin to elucidate the...
Publication Info
- Year
- 1988
- Type
- article
- Volume
- 263
- Issue
- 31
- Pages
- 16051-16054
- Citations
- 128
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
128
OpenAlex
1
Influential
113
CrossRef
Cite This
Hiroshi Maeda,
Yasuhiro Matsumura,
H. Kato
(1988).
Purification and identification of [hydroxyprolyl3]bradykinin in ascitic fluid from a patient with gastric cancer..
Journal of Biological Chemistry
, 263
(31)
, 16051-16054.
https://doi.org/10.1016/s0021-9258(18)37555-0
Identifiers
- DOI
- 10.1016/s0021-9258(18)37555-0
- PMID
- 3182782