Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus

Abstract

Significance Middle East Respiratory Syndrome coronavirus (MERS-CoV) causes severe respiratory disease with a high mortality rate. There is no licensed vaccine or antiviral for MERS. Here we isolated for the first time, to our knowledge, a potent MERS-CoV–neutralizing antibody from memory B cells of an infected individual. This antibody binds to a novel site on the viral Spike protein, neutralizes by interfering with the binding to the cellular receptor CD26, and is highly effective both in prophylaxis and in therapy in a relevant mouse model. This antibody can be developed for prophylaxis, for postexposure prophylaxis, or for the treatment of severe MERS-CoV infections.

Keywords

Middle East respiratory syndrome coronavirusAntibodyVirologyMonoclonal antibodyChinese hamster ovary cellNeutralizing antibodyBiologyCoronavirusImmunologyVero cellMedicineCoronavirus disease 2019 (COVID-19)Cell cultureVirus

MeSH Terms

Amino Acid SequenceAnimalsAntibodiesMonoclonalAntibodiesNeutralizingAntibodiesViralB-LymphocytesBinding SitesCHO CellsCoronavirus InfectionsCricetinaeCricetulusDipeptidyl Peptidase 4Enzyme-Linked Immunosorbent AssayFemaleHumansImmunologic MemoryMaleMiceMiceInbred BALB CMiddle AgedMiddle East Respiratory Syndrome CoronavirusMolecular ConformationMolecular Sequence DataMutationProtein BindingSequence HomologyAmino AcidSpike GlycoproteinCoronavirusViral Vaccines

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Publication Info

Year: 2015
Type: article
Volume: 112
Issue: 33
Pages: 10473-10478
Citations: 235
Access: Closed

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APA Style

                            
                                
                                    Davide Corti, 
                                
                                    Jincun Zhao, 
                                
                                    Mattia Pedotti
                                
                                et al.
                            
                            (2015). 
                            Prophylactic and postexposure efficacy of a potent human monoclonal antibody against MERS coronavirus. 
                            Proceedings of the National Academy of Sciences
                            , 112
                            (33)
                            , 10473-10478.
                            https://doi.org/10.1073/pnas.1510199112
                        

Identifiers

DOI: 10.1073/pnas.1510199112
PMID: 26216974
PMCID: PMC4547275

Data Quality

Data completeness: 86%