Abstract

Messenger RNA (mRNA) stability, localization, and translation are largely determined by sequences in the 3′ untranslated region (3′UTR). We found a conserved increase in expression of mRNAs terminating at upstream polyadenylation sites after activation of primary murine CD4 + T lymphocytes. This program, resulting in shorter 3′UTRs, is a characteristic of gene expression during immune cell activation and correlates with proliferation across diverse cell types and tissues. Forced expression of full-length 3′UTRs conferred reduced protein expression. In some cases the reduction in protein expression could be reversed by deletion of predicted microRNA target sites in the variably included region. Our data indicate that gene expression is coordinately regulated, such that states of increased proliferation are associated with widespread reductions in the 3′UTR-based regulatory capacity of mRNAs.

Keywords

PolyadenylationThree prime untranslated regionUntranslated regionmicroRNAMessenger RNATranslation (biology)BiologyGene expressionGeneMolecular biologyRegulation of gene expressionRNACell biologyGenetics

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Publication Info

Year
2008
Type
article
Volume
320
Issue
5883
Pages
1643-1647
Citations
1330
Access
Closed

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Rickard Sandberg, Joel R. Neilson, Arup Kumar Sarma et al. (2008). Proliferating Cells Express mRNAs with Shortened 3' Untranslated Regions and Fewer MicroRNA Target Sites. Science , 320 (5883) , 1643-1647. https://doi.org/10.1126/science.1155390

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DOI
10.1126/science.1155390