Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked

Abstract

Bcl-2 is an integral membrane protein located mainly on the outer membrane of mitochondria. Overexpression of Bcl-2 prevents cells from undergoing apoptosis in response to a variety of stimuli. Cytosolic cytochrome c is necessary for the initiation of the apoptotic program, suggesting a possible connection between Bcl-2 and cytochrome c, which is normally located in the mitochondrial intermembrane space. Cells undergoing apoptosis were found to have an elevation of cytochrome c in the cytosol and a corresponding decrease in the mitochondria. Overexpression of Bcl-2 prevented the efflux of cytochrome c from the mitochondria and the initiation of apoptosis. Thus, one possible role of Bcl-2 in prevention of apoptosis is to block cytochrome c release from mitochondria.

Keywords

Cytochrome cMitochondrionApoptosomeMitochondrial apoptosis-induced channelApoptosisCell biologyCytosolCytochromeIntermembrane spaceCytochrome bBiologyMitochondrial intermembrane spaceCytochrome C1ChemistryBiochemistryBacterial outer membraneProgrammed cell deathCoenzyme Q – cytochrome c reductaseMitochondrial DNACaspaseGeneEnzyme

MeSH Terms

ApoproteinsApoptosisCaspase 3CaspasesCysteine EndopeptidasesCytochrome c GroupCytochromes cCytosolDNA FragmentationEnzyme ActivationEtoposideHL-60 CellsHeLa CellsHumansIntracellular MembranesMembrane PotentialsMitochondriaPoly(ADP-ribose) PolymerasesProto-Oncogene Proteins c-bcl-2StaurosporineTransfection

Affiliated Institutions

Related Publications

Tumor necrosis factor α induces a caspase-independent death pathway in human neutrophils

Nikolai A. Maianski , Dirk Roos , Taco W. Kuijpers

Tumor necrosis factor α (TNF-α) is a cytokine with multiple roles in the immune system, including the induction and potentiation of cellular functions in neutrophils (PMNs). TNF...

2003 Blood 123 citations

The Metabolic Fate of Mitochondrial Hydrogen Peroxide

Hans Nohl , Werner Jordan

Mitochondrial H 2 O 2 formation is not in equilibrium with defence mechanisms that counteract an accumulation of H 2 , in rat‐heart cell. A model for the accumulation kinetics i...

1980 European Journal of Biochemistry 96 citations

Involvement of Caspases in Neutrophil Apoptosis: Regulation by Reactive Oxygen Species

Bengt Fadeel , Anders Åhlin , Jan‐Inge Henter +2 more

Abstract Human neutrophils have a short half-life and are believed to die by apoptosis or programmed cell death both in vivo and in vitro. We found that caspases are activated i...

1998 Blood 342 citations

bcl-2 inhibits apoptosis of neutrophils but not their engulfment by macrophages.

Eric Lagasse , Irving L. Weissman

Neutrophils, the most common inflammatory leukocytes, have the most limited life span of all blood cells. After they undergo apoptosis, they are recognized and engulfed by macro...

1994 The Journal of Experimental Medicine 280 citations

Purification and properties of cytochrome <italic>c</italic> and two peroxidases from spinach leaves

Kozi Asada , Masaaki Takahashi

1. A simultaneous purification procedure of cytochrome c, peroxidases, ferredoxin, ferredoxin-NADP reductase and sulfite reductase from spinach leaves is described. Cytochrome c...

1971 Plant and Cell Physiology 22 citations

Publication Info

Year: 1997
Type: article
Volume: 275
Issue: 5303
Pages: 1129-1132
Citations: 4912
Access: Closed

External Links

Download PDF (Free) View on DOI.org PubMed Semantic Scholar

Social Impact

Altmetric

Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

4912

OpenAlex

107

Influential

3938

CrossRef

Cite This

APA Style

                            
                                
                                    Jie Yang, 
                                
                                    Xuesong Liu, 
                                
                                    Kapil N. Bhalla
                                
                                et al.
                            
                            (1997). 
                            Prevention of Apoptosis by Bcl-2: Release of Cytochrome c from Mitochondria Blocked. 
                            Science
                            , 275
                            (5303)
                            , 1129-1132.
                            https://doi.org/10.1126/science.275.5303.1129
                        

Identifiers

DOI: 10.1126/science.275.5303.1129
PMID: 9027314

Data Quality

Data completeness: 81%