Abstract

The technic of DNA-DNA hybridization was used for prenatal diagnosis of a pregnancy at risk for homozygous alpha-thalassemia. Fibroblasts were cultured from amniotic fluid, and the number of alpha-globin genes in the DNA was quantified by hybridization with radioactive DNA complementary to alpha-globin mRNA sequences. As compared to control studies of DNA from patients with alpha-thalassemia syndromes and from unaffected subjects, the results indicated that the fetus had alpha-thalassemia-1. The diagnosis was confirmed by umbilical-cord blood studies.

Keywords

Prenatal diagnosisThalassemiaMedicineAmniotic fluidUmbilical cordFetusAlpha (finance)Alpha-thalassemiaHybridization probeDNAPregnancyHemoglobinopathyObstetricsBeta thalassemiaGeneMolecular biologyGeneticsImmunologyInternal medicineHemolytic anemiaBiologyGenotypeSurgery

MeSH Terms

Asian PeopleCaliforniaChinaDNAFemaleGlobinsHomozygoteHumansNucleic Acid HybridizationPregnancyPrenatal DiagnosisThalassemia

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Publication Info

Year
1976
Type
article
Volume
295
Issue
21
Pages
1165-1167
Citations
145
Access
Closed

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Cite This

Yuet Wai Kan, Mitchell S. Golbus, Andrée M. Dozy (1976). Prenatal Diagnosis of α-Thalassemia. New England Journal of Medicine , 295 (21) , 1165-1167. https://doi.org/10.1056/nejm197611182952104

Identifiers

DOI
10.1056/nejm197611182952104
PMID
980019

Data Quality

Data completeness: 81%