Abstract
The endogenous small subunit of the ribulose-1,5-bisphosphate carboxylase gene rbcS and the light-harvesting chlorophyll a/b-binding protein gene ( LHCP ) of pea are expressed in a light-inducible manner and are active mainly in green chloroplast-containing tissue. Chimeric genes under control of the 5′-flanking sequences of the rbcS ss 3.6 or LHCP AB 80 genes from pea were used to study the factors relating to the tissue-specific and light-inducible expression of these nuclear-encoded genes in transgenic tobacco plants. The results show that plastid development plays a crucial role in the activation of expression of these chimeric genes. Particular members of each of the above gene families respond differently to tissue-specific and environmental factors. Furthermore, the light-inducible expression directed by the 5′-flanking sequence of ss 3.6 rbcS gene is not exclusively mediated by phytochrome, but probably is controlled in large part by another photoreceptor.
Keywords
GeneBiologyTransgeneGeneticsChimeric geneChloroplastGene expressionGene familyRuBisCOPlastidNuclear genePhytochromeGenetically modified cropsMolecular biologyGenomeBotany
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Publication Info
- Year
- 1986
- Type
- article
- Volume
- 233
- Issue
- 4759
- Pages
- 34-38
- Citations
- 173
- Access
- Closed
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Cite This
June Simpson,
Marc Van Montagu,
Luís Herrera‐Estrella
(1986).
Photosynthesis-Associated Gene Families: Differences in Response to Tissue-Specific and Environmental Factors.
Science
, 233
(4759)
, 34-38.
https://doi.org/10.1126/science.233.4759.34
Identifiers
- DOI
- 10.1126/science.233.4759.34