Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples

Abstract

Abstract The amount of nuclear DNA extracted from teeth of 279 individual red fox Vulpes vulpes collected over a period spanning the last three decades was determined by quantitative polymerase chain reaction (PCR). Although teeth were autoclaved during initial collection, 73.8% of extracts contained sufficient DNA concentration (> 5 pg/µL) suitable for reliable microsatellite genotyping but the quantity of nuclear DNA decayed significantly over time in a nonlinear pattern. The success of PCR amplification across four examined canine microsatellites over time was dependent on fragment size. By including data from two different tests for human contamination and from frequencies of allelic dropout and false alleles, the methodological constraints of population genetic studies using microsatellite loci amplified from historic DNA are discussed.

Keywords

BiologyMicrosatelliteVulpesGenotypingNuclear DNAPolymerase chain reactionGeneticsPopulationDNAAlleleMolecular biologyGenotypeMitochondrial DNAGeneEcology

Affiliated Institutions

Related Publications

Taq DNA polymerase slippage mutation rates measured by PCR and quasi-likelihood analysis: (CA/GT)n and (A/T)n microsatellites

Dheeraj Shinde

During microsatellite polymerase chain reaction (PCR), insertion-deletion mutations produce stutter products differing from the original template by multiples of the repeat unit...

2003 Nucleic Acids Research 283 citations

Gene Splicing by Overlap Extension: Tailor-Made Genes Using the Polymerase Chain Reaction

Robert M. Horton , Zeling Cai , Steffan N. Ho +1 more

Gene Splicing by Overlap Extension or "gene SOEing" is a PCR-based method of recombining DNA sequences without reliance on restriction sites and of directly generating mutated D...

2013 BioTechniques 1303 citations

Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA

Monique Gardes , Thomas J. White , J. André Fortin +2 more

We used the polymerase chain reaction (PCR) to amplify specific regions of the nuclear and mitochondrial genomes of fungi using DNA extracted from pure cultures as well as that ...

1991 Canadian Journal of Botany 473 citations

Cell-free cloning using φ29 DNA polymerase

Clyde A. Hutchison , Hamilton O. Smith , Cynthia Pfannkoch +1 more

We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by >10 9 -fold, using φ29 DNA polymerase. The principal difficulty wi...

2005 Proceedings of the National Academy o... 172 citations

Correlation of Chest CT and RT-PCR Testing for Coronavirus Disease 2019 (COVID-19) in China: A Report of 1014 Cases

Tao Ai , Zhenlu Yang , Hongyan Hou +6 more

Background Chest CT is used in the diagnosis of coronavirus disease 2019 (COVID-19) and is an important complement to reverse-transcription polymerase chain reaction (RT-PCR) te...

2020 Radiology 5964 citations

Publication Info

Year: 2003
Type: article
Volume: 12
Issue: 4
Pages: 1087-1093
Citations: 107
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

107

OpenAlex

Cite This

APA Style

                            
                                
                                    Peter Wandeler, 
                                
                                    Steve Smith, 
                                
                                    Phillip A. Morin
                                
                                et al.
                            
                            (2003). 
                            Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples. 
                            Molecular Ecology
                            , 12
                            (4)
                            , 1087-1093.
                            https://doi.org/10.1046/j.1365-294x.2003.01807.x
                        

Identifiers

DOI: 10.1046/j.1365-294x.2003.01807.x