Abstract
We have developed a simple method for the fractionation of T and B lymphocytes. Plastic dishes coated with antibodies specific for mouse Ig selectively bind splenic B lymphocytes. The adherent cells are easily removed by gentle pipetting; both adherent and nonadherent populations retain immunologic function. In a typical experiment, when 3 X 10(7) splenic lymphocytes were added to a 100 X 15 mm plastic dish coated with microgram quantities of anti-Ig, 98 % of the nonadherent cells were Ig negative and 97% of the adherent cells were Ig positive. The method is sufficiently sensitive to allow detection and separation of cell types comprising as little as 2% of the total population and can be modified to allow the selection of cells by a double-antibody procedure. We believe that the plastic dish method will be generally useful for fractionating cells on the basis of their cell surface antigens.
Keywords
Panning (audio)AntibodyAntigenPopulationMolecular biologyCellChemistryPipetteMicrogramImmunologyBiologyIn vitroBiochemistryMedicine
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Publication Info
- Year
- 1978
- Type
- article
- Volume
- 75
- Issue
- 6
- Pages
- 2844-2848
- Citations
- 1303
- Access
- Closed
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Cite This
Lawrence J. Wysocki,
Vicki L. Sato
(1978).
"Panning" for lymphocytes: a method for cell selection..
Proceedings of the National Academy of Sciences
, 75
(6)
, 2844-2848.
https://doi.org/10.1073/pnas.75.6.2844
Identifiers
- DOI
- 10.1073/pnas.75.6.2844