Abstract
A molecular ecological study was performed on an Australian soil sample to unravel a substantial portion of the bacterial diversity. A large fragment of the 16S rRNA gene was amplified, using DNA isolated by lysing the microorganisms directly within the soil matrix, and a clone library was generated. Comparative sequence analysis of 30 clones and dot blot hybridization of 83 additional clones with defined oligonucleotide probes revealed the presence of three major groups of prokaryotes of the domain Bacteria. The first one comprises 57 clones that indicate relatives of nitrogen-fixing bacteria of the alpha-2 subclass of the class Proteobacteria; the second group of 7 clones originates from members of the order Planctomycetales that, however, reveal no close relationship to any of the described Planctomycetales species; 22 clones of the third group are indicative of members of a novel main line of descent, sharing a common ancestry with members of planctomycetes and chlamydiae.
Keywords
BiologyBacteriaGeneticsProteobacteriaLibrarySequence analysis16S ribosomal RNAOligonucleotideMicrobiologyDNA
Affiliated Institutions
Related Publications
Search for the optimal sequence of the ribosome binding site by random oligonucleotide-directed mutagenesis
Synthetic DNA duplexes corresponding to the ribosome binding site (RBS) were synthesized through the phosphite method on solid support. The synthetic RBS DNA with partial random...
HD-Zip proteins: members of an Arabidopsis homeodomain protein superfamily.
Homeobox genes encode a large family of homeodomain proteins in animal systems. To test whether such genes are also abundant in higher plants, degenerate oligonucleotides comple...
Dominant and diet-responsive groups of bacteria within the human colonic microbiota
Abstract The populations of dominant species within the human colonic microbiota can potentially be modified by dietary intake with consequences for health. Here we examined the...
Classification and Diagnosis of Diabetes Mellitus and Other Categories of Glucose Intolerance
A classification of diabetes and other categories of glucose intolerance, based on contemporary knowledge of this heterogeneous syndrome, was developed by an international workg...
Identification of a second transmembrane protein tyrosine phosphatase, IA-2beta, as an autoantigen in insulin-dependent diabetes mellitus: precursor of the 37-kDa tryptic fragment.
A novel cDNA, IA-2beta, was isolated from a mouse neonatal brain library. The predicted protein sequence revealed an extracellular domain, a transmembrane region, and an intrace...
Publication Info
- Year
- 1992
- Type
- article
- Volume
- 174
- Issue
- 15
- Pages
- 5072-5078
- Citations
- 430
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
430
OpenAlex
Cite This
Werner Liesack,
Erko Stackebrandt
(1992).
Occurrence of novel groups of the domain Bacteria as revealed by analysis of genetic material isolated from an Australian terrestrial environment.
Journal of Bacteriology
, 174
(15)
, 5072-5078.
https://doi.org/10.1128/jb.174.15.5072-5078.1992
Identifiers
- DOI
- 10.1128/jb.174.15.5072-5078.1992