Abstract
Thermodynamics of 22 oligonucleotides with internal single G.A mismatches dissolved in 1 M NaCl were determined from absorbance versus temperature melting curves. These data, combined with five literature sequences, were used to derive nearest-neighbor thermodynamic parameters for seven linearly independent trimer sequences with internal G.A mismatches and Watson-Crick flanking base pairs. The G.A mismatch parameters predict DeltaG degrees 37, DeltaH degrees, DeltaS degrees, and TM with average deviations of 4.4%, 7.4%, 8.0%, and 1.5 degrees C, respectively. The nearest-neighbor parameters show that G.A mismatch stability is strongly context dependent, and DeltaG degrees 37 ranges from +1.16 kcal/mol for TGA/AAT to -0.78 kcal/mol for GGC/CAG. In addition, one-dimensional 1H NMR spectra show that the G.A pairing geometry is pH and context dependent.
Keywords
Context (archaeology)Trimerk-nearest neighbors algorithmChemistryThermodynamicsBase pairChemical stabilityBase (topology)CrystallographyDNAPhysicsMathematicsBiochemistryOrganic chemistry
Affiliated Institutions
Related Publications
Three Approaches to Qualitative Content Analysis
Content analysis is a widely used qualitative research technique. Rather than being a single method, current applications of content analysis show three distinct approaches: con...
Toward a psychology of positive youth development.
This article analyzes the development of initiative as an exemplar of one of many learning experiences that should be studied as part of positive youth development. The capacity...
The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research
Reproducible science requires transparent reporting. The ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments) were originally developed in 2010 to improve the r...
Alliance Capitalism
Business practices in Japan inspire fierce and even acrimonious debate, especially when they are compared to American practices. This book attempts to explain the remarkable eco...
Publication Info
- Year
- 1998
- Type
- article
- Volume
- 37
- Issue
- 8
- Pages
- 2170-2179
- Citations
- 242
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
242
OpenAlex
Cite This
Hatim T. Allawi,
John SantaLucia
(1998).
Nearest Neighbor Thermodynamic Parameters for Internal G·A Mismatches in DNA.
Biochemistry
, 37
(8)
, 2170-2179.
https://doi.org/10.1021/bi9724873
Identifiers
- DOI
- 10.1021/bi9724873