Abstract
A monoclonal antibody derived by fusion of mouse myeloma cells with spleen cells from a mouse immunized with F9 teratocarcinoma cells is described. This antibody, which reacts with embryonal carcinoma cells of mouse and human origin and with some preimplantation stage mouse embryos, defines an embryonic stage-specific antigen. This stage-specific antigen (SSEA-1) is first detected on blastomeres of 8-cell stage embryos. Trophectodermal cells are transitorily positive; however, each cell in the inner cell mass eventually expresses this antigen.
Keywords
Embryonic stem cellAntigenMonoclonal antibodyBiologyMolecular biologyTeratocarcinomaSpleenAntibodyEmbryoBlastomereCell biologyCellular differentiationImmunologyEmbryogenesisGenetics
Related Publications
Snail is an immediate early target gene of parathyroid hormone related peptide signaling in parietal endoderm formation.
In mouse development, parietal endoderm (PE) is formed from both primitive endoderm (PrE) and visceral endoderm (VE). This process can be mimicked in vitro by using F9 embryonal...
The role of E-cadherin and integrins in mesoderm differentiation and migration at the mammalian primitive streak
ABSTRACT We have examined the role of cell-cell and cell-extracellular matrix (ECM) interactions during mesoderm differentiation and migration at the primitive streak of the mou...
<i>nodal</i> expression in the primitive endoderm is required for specification of the anterior axis during mouse gastrulation
ABSTRACT Mouse nodal, a member of the TGFβ family of secreted growth factors is essential for gastrulation. We recently generated a nodallacZ reporter allele by homologous recom...
Overexpression of <i>NANOG</i> in human ES cells enables feeder-free growth while inducing primitive ectoderm features
Human embryonic stem cells (HESCs) are pluripotent cells derived from the ICM of blastocyst stage embryos. As the factors needed for their growth are largely undefined, they are...
The Mouse Snail Gene Encodes a Key Regulator of the Epithelial-Mesenchymal Transition
Snail family genes encode DNA binding zinc finger proteins that act as transcriptional repressors. Mouse embryos deficient for the Snail (Sna) gene exhibit defects in the format...
Publication Info
- Year
- 1978
- Type
- article
- Volume
- 75
- Issue
- 11
- Pages
- 5565-5569
- Citations
- 1296
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
1296
OpenAlex
Cite This
Davor Solter,
Barbara B. Knowles
(1978).
Monoclonal antibody defining a stage-specific mouse embryonic antigen (SSEA-1)..
Proceedings of the National Academy of Sciences
, 75
(11)
, 5565-5569.
https://doi.org/10.1073/pnas.75.11.5565
Identifiers
- DOI
- 10.1073/pnas.75.11.5565