Mis-splicing yields circular RNA molecules.

Claude Cocquerelle; Bénédicte Mascrez; Dominique Hétuin; Bernard Bailleul

doi:10.1096/fasebj.7.1.7678559

Abstract

We previously identified novel human ets-1 transcripts in which the normal order of exons is inverted, and demonstrated that although the order of exons is different than in the genomic DNA, splicing of these exons out of order occurs in pairs using genuine splice sites (1). Here we determine the structure of these novel transcripts, showing that they correspond to circular RNA molecules containing only exons in genomic order. These transcripts are stable molecules, localized in the cytoplasmic component of the cells. To our knowledge, this is the first case of circular transcripts being processed from nuclear pre-mRNA in eukaryotes. This new type of transcript might represent a novel aspect of gene expression and hold some interesting clues about the splicing mechanism.

Keywords

ExonRNA splicingCircular RNARNAAlternative splicingGeneGeneticsgenomic DNABiologyExonic splicing enhancerDNATrans-splicingSplicing factorMolecular biology

Affiliated Institutions

Related Publications

Exon circularization in mammalian nuclear extracts.

Zvi Pasman , Michael D. Been , Mariano A. García-Blanco

Correct ligation of exons in pre-mRNA splicing requires splice site juxtaposition (splice site pairing), usually involving a 5' splice site and a downstream 3' splice site. Spli...

1996 PubMed 188 citations

Inverted repeats are necessary for circularization of the mouse testis Sry transcript

Robert Dubin , Manija A. Kazmi , Harry Ostrer

Circular non-polyadenylated RNA molecules have been identified as stable transcription products of the human ETS-1 and mouse Sry genes. RNA circularization has been proposed to ...

1995 Gene 156 citations

Circular RNAs Are the Predominant Transcript Isoform from Hundreds of Human Genes in Diverse Cell Types

Julia Salzman , Charles Gawad , Peter Lincoln Wang +2 more

Most human pre-mRNAs are spliced into linear molecules that retain the exon order defined by the genomic sequence. By deep sequencing of RNA from a variety of normal and maligna...

2012 PLoS ONE 2532 citations

A Global View of Gene Activity and Alternative Splicing by Deep Sequencing of the Human Transcriptome

Marc Sultan , Marcel H. Schulz , Hugues Richard +13 more

The functional complexity of the human transcriptome is not yet fully elucidated. We report a high-throughput sequence of the human transcriptome from a human embryonic kidney a...

2008 Science 1294 citations

Noisy Splicing Drives mRNA Isoform Diversity in Human Cells

Joseph K. Pickrell , Athma A. Pai , Yoav Gilad +1 more

While the majority of multiexonic human genes show some evidence of alternative splicing, it is unclear what fraction of observed splice forms is functionally relevant. In this ...

2010 PLoS Genetics 318 citations

Publication Info

Year: 1993
Type: article
Volume: 7
Issue: 1
Pages: 155-160
Citations: 1261
Access: Closed

External Links

View on DOI.org

Social Impact

Altmetric

Mis-splicing yields circular RNA molecules.

PlumX Metrics

Social media, news, blog, policy document mentions

Citation Metrics

1261

OpenAlex

Cite This

APA Style

                            
                                
                                    Claude Cocquerelle, 
                                
                                    Bénédicte Mascrez, 
                                
                                    Dominique Hétuin
                                
                                et al.
                            
                            (1993). 
                            Mis-splicing yields circular RNA molecules.. 
                            The FASEB Journal
                            , 7
                            (1)
                            , 155-160.
                            https://doi.org/10.1096/fasebj.7.1.7678559
                        

Identifiers

DOI: 10.1096/fasebj.7.1.7678559