Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

Abstract

Abstract Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of origin and change the functions and phenotypes of other cells. These features indicate strong biomarker and therapeutic potential and have generated broad interest, as evidenced by the steady year‐on‐year increase in the numbers of scientific publications about EVs. Important advances have been made in EV metrology and in understanding and applying EV biology. However, hurdles remain to realising the potential of EVs in domains ranging from basic biology to clinical applications due to challenges in EV nomenclature, separation from non‐vesicular extracellular particles, characterisation and functional studies. To address the challenges and opportunities in this rapidly evolving field, the International Society for Extracellular Vesicles (ISEV) updates its ‘Minimal Information for Studies of Extracellular Vesicles’, which was first published in 2014 and then in 2018 as MISEV2014 and MISEV2018, respectively. The goal of the current document, MISEV2023, is to provide researchers with an updated snapshot of available approaches and their advantages and limitations for production, separation and characterisation of EVs from multiple sources, including cell culture, body fluids and solid tissues. In addition to presenting the latest state of the art in basic principles of EV research, this document also covers advanced techniques and approaches that are currently expanding the boundaries of the field. MISEV2023 also includes new sections on EV release and uptake and a brief discussion of in vivo approaches to study EVs. Compiling feedback from ISEV expert task forces and more than 1000 researchers, this document conveys the current state of EV research to facilitate robust scientific discoveries and move the field forward even more rapidly.

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Affiliated Institutions

• University of Gothenburg SE
• Instituto de Ciências Farmacêuticas BR
• Trinity College Dublin IE
• Agency for Science, Technology and Research SG
• University Hospital of Basel CH
• Johns Hopkins Medicine US
• University of Ljubljana SI
• National University of Singapore SG
• AstraZeneca (Brazil) BR
• National Cancer Institute US
• Southern Medical University CN
• John Radcliffe Hospital GB
• Paracelsus Medical University AT
• Amsterdam University Medical Centers NL
• University of California, Davis US
• Salzburger Landeskliniken AT
• Beckman Research Institute
• University of Turin IT
• Tokyo Medical University JP
• Icahn School of Medicine at Mount Sinai US
• Flinders University AU
• Cedars-Sinai Medical Center US
• University of Amsterdam NL
• Ikerbasque ES
• City of Hope US
• Institute of Molecular and Cell Biology SG
• Utrecht University NL
• Institut Curie FR
• University of Virginia Health System US
• QIMR Berghofer Medical Research Institute AU
• University Medical Center Utrecht NL
• University of Brescia IT
• Institut Pasteur de Montevideo UY
• Vrije Universiteit Amsterdam NL
• Semmelweis University HU
• University of Basel CH
• Oregon Health & Science University US
• Colorado State University US
• Consorzio Interuniversitario per lo Sviluppo dei Sistemi a Grande Interfase IT
• Victoria University AU
• Université Paris Sciences et Lettres FR
• Cancer Research Institute Ghent BE
• Royan Institute IR
• Institute of Nanostructured Materials IT
• Erasmus MC Cancer Institute NL
• Northeastern University US
• Sun Yat-sen University CN
• University of Oxford GB
• International Society of Nephrology BE
• HUN-REN Research Centre for Natural Sciences HU
• Institute of Materials and Environmental Chemistry HU
• University of Georgia US
• National Institutes of Health US
• All India Institute of Medical Sciences IN
• Thomas Jefferson University US
• Nanfang Hospital CN
• Ghent University BE
• University of Denver US
• The First Affiliated Hospital, Sun Yat-sen University CN
• MRC Weatherall Institute of Molecular Medicine GB
• Inserm FR
• Michigan Science Center US
• CIC bioGUNE ES
• Universidade Federal de São Paulo BR
• Sahlgrenska University Hospital SE
• Ottawa Hospital Research Institute
• The Ohio State University US
• Chinese University of Hong Kong HK
• TauRx Pharmaceuticals (Singapore) SG
• Academic Center for Education, Culture and Research IR
• University of Auckland NZ
• Johns Hopkins University US
• University of Ottawa CA
• Vanderbilt University Medical Center US

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