Abstract

We have developed a novel microfluidic device constructed from poly(dimethylsiloxane) using multilayer soft lithography technology for the analysis of single cells. The microfluidic network enables the passive and gentle separation of a single cell from the bulk cell suspension, and integrated valves and pumps enable the precise delivery of nanoliter volumes of reagents to that cell. Various applications are demonstrated, including cell viability assays, ionophore-mediated intracellular Ca2+ flux measurements, and multistep receptor-mediated Ca2+ measurements. These assays, and others, are achieved with significant improvements in reagent consumption, analysis time, and temporal resolution over macroscale alternatives.

Keywords

MicrofluidicsChemistrySingle-cell analysisReagentSoft lithographyIonophoreNanotechnologyCellIntracellularResolution (logic)MembraneMaterials scienceBiochemistryComputer science

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Publication Info

Year
2003
Type
article
Volume
75
Issue
14
Pages
3581-3586
Citations
595
Access
Closed

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Aaron R. Wheeler, William Throndset, Rebecca J. Whelan et al. (2003). Microfluidic Device for Single-Cell Analysis. Analytical Chemistry , 75 (14) , 3581-3586. https://doi.org/10.1021/ac0340758

Identifiers

DOI
10.1021/ac0340758