Abstract
We have used a modification of the Berk-Sharp technique to determine that the 5' termini of the mouse 15 S beta-globin precursor and the mature mRNA have identical map coordinates. The modification involves the use of 5' (or 3') terminally labeled probes; it allows the detection of the precursor in the presence of excess mature mRNA.
Keywords
BiologyGlobinMessenger RNAMolecular biologyRNAGeneticsGene
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Publication Info
- Year
- 1979
- Type
- article
- Volume
- 7
- Issue
- 5
- Pages
- 1175-1194
- Citations
- 1286
- Access
- Closed
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Cite This
Robert F. Weaver,
Charles Weissmann
(1979).
Mapping of RNA by a modification of the Berk-Sharp procedure: the 5′ termini of 15 S β-globin mRNA precursor and mature 10 S β-globin mRNA have identical map coordinates.
Nucleic Acids Research
, 7
(5)
, 1175-1194.
https://doi.org/10.1093/nar/7.5.1175
Identifiers
- DOI
- 10.1093/nar/7.5.1175