Abstract
We hypothesize that loss of imprinting (LOI) of the insulin-like growth factor II ( IGF2 ) gene is associated with a predisposition to sporadic colorectal cancer. We confirmed a previously known strong correlation between LOI and microsatellite instability and showed that LOI was not a consequence of microsatellite instability or mismatch repair deficiency. LOI of IGF2 correlated strongly with biallelic hypermethylation of a core of five CpG sites in the insulator region of IGF2/H19 , which is a known CTCF-binding element. As this methylation-dependent LOI was present in both tumors and normal colonic mucosa, it is possible that hypermethylation creates a field defect predisposing to cancer.
Keywords
Microsatellite instabilityImprinting (psychology)CTCFDNA methylationBiologyCpG siteGenomic imprintingCancer researchInsulin-like growth factor 2MethylationColorectal cancerDNA mismatch repairMolecular biologyCancerGeneticsGeneTranscription factorMicrosatelliteAlleleGene expressionEnhancer
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Publication Info
- Year
- 2000
- Type
- article
- Volume
- 98
- Issue
- 2
- Pages
- 591-596
- Citations
- 227
- Access
- Closed
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Cite This
Hidewaki Nakagawa,
Robert B. Chadwick,
Païvi Peltomäki
et al.
(2000).
Loss of imprinting of the insulin-like growth factor II gene occurs by biallelic methylation in a core region of <i>H19</i> -associated CTCF-binding sites in colorectal cancer.
Proceedings of the National Academy of Sciences
, 98
(2)
, 591-596.
https://doi.org/10.1073/pnas.98.2.591
Identifiers
- DOI
- 10.1073/pnas.98.2.591