Abstract

1.This study investigated the synergistic effect of L-arginine (iNOS substrate) combined with 5-fluorouracil (5-FU, iNOS inducer) on epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma (HCC).2.In vitro, the combination significantly inhibited HCC cell proliferation, invasion, migration, and upregulated iNOS. It also decreased mesenchymal markers (N-cadherin, vimentin, Snail, Slug) and increased epithelial E-cadherin.3.In vivo, using DEN-induced HCC rats, the combination group showed extensive tumor necrosis, reduced mitoses, enhanced iNOS, reduced mesenchymal markers, elevated E-cadherin, fewer pseudopodia, and increased cytoplasmic vacuolation compared to the model group.4.Thus, L-arginine + 5-FU synergistically inhibits HCC metastasis by suppressing EMT via iNOS upregulation.

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Year
2025
Type
article
Pages
1-12
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0
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Han Wang, Yao Lu, Shuhua Cheng et al. (2025). L-arginine combined with 5-fluorouracil inhibits EMT by regulating iNOS expression in hepatocellular carcinoma. Xenobiotica , 1-12. https://doi.org/10.1080/00498254.2025.2599958

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DOI
10.1080/00498254.2025.2599958