Abstract
The formation of knotted species on random ring closure of two DNAs that are 5.6 kilobase pairs (kbp) and 8.6 kbp in length was measured, and these data were used to calculate the effective DNA helix diameter as a function of sodium ion and magnesium ion concentration. In the presence of more than 50 mM magnesium ion, interactions between DNA segments appear to be attractive rather than repulsive. The free energy of formation of relaxed trefoil and figure-eight DNA knots and of supercoiled trefoil DNA knots was also evaluated.
Keywords
TrefoilDNABiophysicsDNA supercoilSodiumRing (chemistry)ChemistryCrystallographyBiologyBiochemistryDNA replication
Affiliated Institutions
Related Publications
SUPERCOILED CIRCULAR DNA-PROTEIN COMPLEX IN <i>Escherichia coli</i> : PURIFICATION AND INDUCED CONVERSION TO AN OPEN CIRCULAR DNA FORM
The 23 S twisted circular form of Col E 1 DNA has been isolated from Escherichia coli as a tightly associated DNA-protein complex with a sedimentation coefficient of approximate...
Publication Info
- Year
- 1993
- Type
- article
- Volume
- 260
- Issue
- 5107
- Pages
- 533-536
- Citations
- 289
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
289
OpenAlex
Cite This
Stanley Y. Shaw,
James C. Wang
(1993).
Knotting of a DNA Chain During Ring Closure.
Science
, 260
(5107)
, 533-536.
https://doi.org/10.1126/science.8475384
Identifiers
- DOI
- 10.1126/science.8475384