Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease

Abstract

Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of alpha-amylase messenger ribonucleic acid.

Keywords

RNARibonucleaseRNase PChemistryBiochemistryHomogenization (climate)Pancreatic ribonucleaseMolecular biologyBiology

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Publication Info

Year: 1979
Type: article
Volume: 18
Issue: 24
Pages: 5294-5299
Citations: 22201
Access: Closed

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APA Style

                            
                                
                                    John M. Chirgwin, 
                                
                                    Alan Przybyla, 
                                
                                    Raymond J. MacDonald
                                
                                et al.
                            
                            (1979). 
                            Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease. 
                            Biochemistry
                            , 18
                            (24)
                            , 5294-5299.
                            https://doi.org/10.1021/bi00591a005
                        

Identifiers

DOI: 10.1021/bi00591a005