Abstract
The autoxidation of pyrogallol was investigated in the presence of EDTA in the pH range 7.9–10.6. The rate of autoxidation increases with increasing pH. At pH 7.9 the reaction is inhibited to 99% by superoxide dismutase, indicating an almost total dependence on the participation of the superoxide anion radical, O 2 · − , in the reaction. Up to pH 9.1 the reaction is still inhibited to over 90% by superoxide dismutase, but at higher alkalinity, O 2 · − ‐independent mechanisms rapidly become dominant. Catalase has no effect on the autoxidation but decreases the oxygen consumption by half, showing that H 2 O 2 is the stable product of oxygen and that H 2 O 2 is not involved in the autoxidation mechanism. A simple and rapid method for the assay of superoxide dismutase is described, based on the ability of the enzyme to inhibit the autoxidation of pyrogallol. A plausible explanation is given for the non‐competitive part of the inhibition of catechol O ‐methyltransferase brought about by pyrogallol.
Keywords
AutoxidationPyrogallolChemistrySuperoxide dismutaseSuperoxideCatecholCatalaseHomolysisOxygenMedicinal chemistryRadicalEnzymePhotochemistryNuclear chemistryBiochemistryOrganic chemistry
Affiliated Institutions
Related Publications
The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase
The rate of autoxidation of epinephrine and the sensitivity of this autoxidation to inhibition by superoxide dismutase were both augmented, as the pH was raised from 7.8 → 10.2....
6-Hydroxydopamine: Evidence for Superoxide Radical as an Oxidative Intermediate
Superoxide dismutase inhibited the autoxidation of 6-hydroxydopamine as measured by the rate of formation of a quinone and the rate of oxygen consumption. These observations dem...
THE ROLE OF O<sub>2</sub><sup>‐</sup> IN THE CHEMILUMINESCENCE OF LUMINOL*
Abstract— The chemiluminescence of luminol in buffered aqueous solutions is inhibited by superoxide dismutase. This occurs whether the luminescence is induced by ferricyanide, p...
The Utility of Superoxide Dismutase in Studying Free Radical Reactions
The superoxide dismutase (dismutase) which has already been found to be useful in detecting the role of O2·- in certain enzymic reactions has here been shown to be applicable to...
Influence of Hydrogen Peroxide upon Carbon Dioxide Photoassimilation in the Spinach Chloroplast
Photosynthesis and the Warburg effect (O(2) inhibition of photosynthesis) were evaluated in preparations of intact spinach chloroplasts enriched with varying amounts of lysed ch...
Publication Info
- Year
- 1974
- Type
- article
- Volume
- 47
- Issue
- 3
- Pages
- 469-474
- Citations
- 9982
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
9982
OpenAlex
Cite This
Stefan L. Marklund,
Gudrun MARKLUND
(1974).
Involvement of the Superoxide Anion Radical in the Autoxidation of Pyrogallol and a Convenient Assay for Superoxide Dismutase.
European Journal of Biochemistry
, 47
(3)
, 469-474.
https://doi.org/10.1111/j.1432-1033.1974.tb03714.x
Identifiers
- DOI
- 10.1111/j.1432-1033.1974.tb03714.x