Abstract
Phage Mu-1 and a thermoinducible derivative, Mu-1 cts 62 were inserted into the broad host range R factor RP4. These hybrid plasmids were transferred by conjugation to a phytopathogenic bacterium Pseudomonas solanacearum GMI 1000 and a legume-root nodule bacterium Rhizobium meliloti 2011. The Mu genome is transcribed and tranlated in these new hosts: P. solanacearum (RP4:Mu cts) cultures have a spontaneous production of about 5 X 10(5) plaque-forming units ml-1 which is similar to the frequency of spontaneous Mu production in E. coli; the Mu production of R. meliloti is lower (about 10(2) plaque-forming units ml-1).
Keywords
RhizobiumBacteriophagePseudomonasPlasmidMicrobiologyBacteriaBiologyEscherichia coliGeneticsGene
Related Publications
R Factor Transfer in Rhizobium leguminosarum
SUMMARY: R factors of the compatibility class P were transferred between strains of Escherichia coli k12 and Rhizobium leguminosarum. These R factors were stable in R. leguminos...
Localization of symbiotic mutations in Rhizobium meliloti
A total of 5 Nod- and 57 Fix- symbiotic mutants of Rhizobium meliloti strain 41 have been isolated after either nitrosoguanidine or Tn5 transposition mutagenesis. Chromosomal lo...
Physical and genetic characterization of symbiotic and auxotrophic mutants of Rhizobium meliloti induced by transposon Tn5 mutagenesis
We have physically and genetically characterized 20 symbiotic and 20 auxotrophic mutants of Rhizobium meliloti, the nitrogen-fixing symbiont of alfalfa (Medicago sativa), isolat...
Publication Info
- Year
- 1977
- Type
- article
- Volume
- 98
- Issue
- 1
- Pages
- 253-263
- Citations
- 67
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
67
OpenAlex
Cite This
Christian Boucher,
B. Bergeron,
Mirta Barate de Bertalmio
et al.
(1977).
Introduction of Bacteriophage Mu into Pseudomonas solanacearum and Rhizobium meliloti using the R Factor RP4.
Journal of General Microbiology
, 98
(1)
, 253-263.
https://doi.org/10.1099/00221287-98-1-253
Identifiers
- DOI
- 10.1099/00221287-98-1-253