Induction of Cytokine Production from Human Monocytes Stimulated with Alginate

Abstract

Alginates are polysaccharides with gel-forming properties composed of 1,4-linked beta-D-mannuronic acid (M), alpha-L-guluronic acid (G), and alternating (MG) blocks. Alginate can be used as a matrix for implanted cells in vivo. In this study, we have examined the ability of alginates and their components to stimulate human monocytes to produce tumor necrosis factor-alpha, interleukin-6, and interleukin-1. Alginates stimulated the monocytes to produce high levels of all three cytokines. Low G alginates were approximately 10 times more potent in inducing cytokine production compared with high G alginates. The M-blocks and the MG-blocks, but not the G-blocks, stimulated the cytokine production. The results demonstrate that the mannuronic acid residues are the active cytokine inducers in alginates.

Keywords

CytokinePolysaccharideTumor necrosis factor alphaIn vitroIn vivoChemistryMonocyteInterleukinBiochemistryImmunologyBiologyBiotechnology

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Publication Info

Year: 1991
Type: article
Volume: 10
Issue: 4
Pages: 286-291
Citations: 387
Access: Closed

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APA Style

                            
                                
                                    Marit Otterlei, 
                                
                                    Kjetill Østgaard, 
                                
                                    Gudmund Skjåk‐Bræk
                                
                                et al.
                            
                            (1991). 
                            Induction of Cytokine Production from Human Monocytes Stimulated with Alginate. 
                            Journal of Immunotherapy
                            , 10
                            (4)
                            , 286-291.
                            https://doi.org/10.1097/00002371-199108000-00007
                        

Identifiers

DOI: 10.1097/00002371-199108000-00007