Abstract
A group of single-stranded d(TC)n specific binding proteins has been detected in the nuclear extracts of several mammalian species that included mouse, human, African green monkey, chimpanzee, and Chinese muntjac. Southwestern analysis of 500 mM KCI nuclear extracts has shown that these proteins cluster in a similar size range, 55.5 to 57 kD. An additional 54 kD band was present for the three primate species examined. The single-stranded d(TC)n binding activity was confirmed with bandshift assay. Specific double-stranded binding activity for duplex d(TC)n.d(GA)n or single-stranded d(GA)n was not detected. The conservation of size distribution and d(TC)n-binding activity across the species examined indicates that this class of single-stranded binding proteins may have an important biological function in vivo.
Keywords
BiologyDNA-binding proteinBinding siteMolecular biologyBiochemistryTranscription factorGene
Affiliated Institutions
Related Publications
Interaction of R17 Coat Protein With Its RNA Binding Site For Translational Repression
The interaction between bacteriophage R17 coat protein and its RNA binding site for translational repression was studied as an example of a sequence-specific RNA-protein interac...
Specific cleavage analysis of mammalian mitochondrial DNA.
Mitochondrial DNA from several mammalian species has been digested with a site-specific restriction endonuclease (HaeIII) from Haemophilus aegyptius. A quantitative analysis of ...
Patterns of nuclear DNA degeneration over time — a case study in historic teeth samples
Abstract The amount of nuclear DNA extracted from teeth of 279 individual red fox Vulpes vulpes collected over a period spanning the last three decades was determined by quantit...
Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA
We used the polymerase chain reaction (PCR) to amplify specific regions of the nuclear and mitochondrial genomes of fungi using DNA extracted from pure cultures as well as that ...
Redox Regulation of Fos and Jun DNA-Binding Activity in Vitro
The proto-oncogenes c- fos and c- jun function cooperatively as inducible transcription factors in signal transduction processes. Their protein products, Fos and Jun, form a het...
Publication Info
- Year
- 1991
- Type
- article
- Volume
- 19
- Issue
- 4
- Pages
- 949-953
- Citations
- 59
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
59
OpenAlex
Cite This
Harris A. Yee,
Alexander Wong,
J.H. van de Sande
et al.
(1991).
Identification of novel single-stranded d(TC)<sub>n</sub>binding proteins in several mammalian species.
Nucleic Acids Research
, 19
(4)
, 949-953.
https://doi.org/10.1093/nar/19.4.949
Identifiers
- DOI
- 10.1093/nar/19.4.949