Abstract
We have examined the consequences of DNA distortion and specific histone-DNA contacts within the nucleosome for integration mediated by the human immunodeficiency virus (HIV)-encoded integrase enzyme. We find that sites of high-frequency integration cluster in the most severely deformed, kinked DNA regions within the nucleosome core. This may reflect either a preference for a wide major groove for association of the integrase or a requirement for target DNA distortion in the DNA strand transfer mechanism. Both the distortion and folding of the target DNA through packaging into nucleosomes may influence the selection of HIV integration sites within the chromosome.
Keywords
IntegraseNucleosomeDNABiologyHistoneGenetics
Affiliated Institutions
Related Publications
Histone acetylation and an epigenetic code
The enzyme-catalyzed acetylation of the N-terminal tail domains of core histones provides a rich potential source of epigenetic information. This may be used both to mediate tra...
Defining the Sequence Specificity of DNA-Binding Proteins by Selecting Binding Sites from Random-Sequence Oligonucleotides: Analysis of Yeast GCN4 Protein
We describe a new method for accurately defining the sequence recognition properties of DNA-binding proteins by selecting high-affinity binding sites from random-sequence DNA. T...
Histone Acetyltransferases
▪ Abstract Transcriptional regulation in eukaryotes occurs within a chromatin setting and is strongly influenced by nucleosomal barriers imposed by histone proteins. Among the w...
Antigenic and Immunogenic Characterization of Recombinant Baculovirus-Expressed Severe Acute Respiratory Syndrome Coronavirus Spike Protein: Implication for Vaccine Design
ABSTRACT The spike (S) glycoprotein of severe acute respiratory syndrome coronavirus (SARS-CoV) mediates the receptor interaction and immune recognition and is considered a majo...
Author response: DNA deaminases induce break-associated mutation showers with implication of APOBEC3B and 3A in breast cancer kataegis
Article Figures and data Abstract eLife digest Introduction Results Discussion Materials and methods References Decision letter Author response Article and author information Me...
Publication Info
- Year
- 1994
- Type
- article
- Volume
- 91
- Issue
- 13
- Pages
- 5913-5917
- Citations
- 237
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
237
OpenAlex
Cite This
Dmitry Pruss,
Frederic D. Bushman,
A P Wolffe
(1994).
Human immunodeficiency virus integrase directs integration to sites of severe DNA distortion within the nucleosome core..
Proceedings of the National Academy of Sciences
, 91
(13)
, 5913-5917.
https://doi.org/10.1073/pnas.91.13.5913
Identifiers
- DOI
- 10.1073/pnas.91.13.5913