Highly Multiplexed Subcellular RNA Sequencing in Situ

Je Hyuk Lee; Evan R Daugharthy; Jonathan Scheiman; Reza Kalhor; Joyce Yang; Thomas C. Ferrante; Richard Terry; Sauveur S. F. Jeanty; Chao Li; Ryoji Amamoto; Derek Peters; Brian M. Turczyk; Adam Marblestone; Samuel A. Inverso; Amy Bernard; Prashant Mali; Xavier Rios; John Aach; George M. Church

doi:10.1126/science.1250212

Abstract

Transcripts Visualized in Situ Despite advances, current methods for single-cell sequencing are unable to resolve transcript location within the cell, so Lee et al. (p. 1360 , published online 27 February) developed a method of fluorescent in situ RNA sequencing (FISSEQ) that works in vivo to show messenger RNA localization within cells. The method amplifies complementary DNA targets by rolling circle amplification, and then in situ cross-linking locks amplicons to produce ample, highly localized templates for three-dimensional sequencing. The technique was tested in fibroblasts to reveal the differences between individual cells during wound repair.

Keywords

In situRNAComputational biologyMultiplexingBiologyChemistryComputer scienceGeneticsGeneTelecommunications

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Publication Info

Year: 2014
Type: article
Volume: 343
Issue: 6177
Pages: 1360-1363
Citations: 988
Access: Closed

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APA Style

                            
                                
                                    Je Hyuk Lee, 
                                
                                    Evan R Daugharthy, 
                                
                                    Jonathan Scheiman
                                
                                et al.
                            
                            (2014). 
                            Highly Multiplexed Subcellular RNA Sequencing in Situ. 
                            Science
                            , 343
                            (6177)
                            , 1360-1363.
                            https://doi.org/10.1126/science.1250212
                        

Identifiers

DOI: 10.1126/science.1250212