Abstract
The DNA sequences of four promoters for the bacteriophage-specified T7 RNA polymerase have been determined. All four regions contain an identical 23 bp sequence 5' pTAATACGACTCACTATAGGGAG-A(oh) which includes the 17 bp prior to and the first 6 bp of the transcribed region. They are located at 46.5, 55, 57 and 87% on the standard T7 map, thereby corresponding to the in vitro T7 class III RNA species IIIa, IV, V and IIIb, respectively. The precise RNA initiation sites were determined by 5' terminal RNA sequence analysis of the transcripts synthesized from promoter-containing DNA fragments. The comparative properties of these promoters for the monomeric T7 RNA polymerase and those for the multimeric E. coli RNA polymerase are discussed.
Keywords
BiologyT7 RNA polymerasePromoterRNA polymerasePolymeraseMolecular biologyRNARNA-dependent RNA polymeraseRNA polymerase IRNA polymerase IIRNA polymerase IIIDNAGeneticsBacteriophageGeneGene expressionEscherichia coli
MeSH Terms
Base SequenceBinding SitesColiphagesDNAViralDNA-Directed RNA PolymerasesGenesRegulatorTranscriptionGenetic
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Publication Info
- Year
- 1979
- Type
- article
- Volume
- 16
- Issue
- 4
- Pages
- 815-825
- Citations
- 87
- Access
- Closed
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Cite This
Margaret D. Rosa
(1979).
Four T7 RNA polymerase promoters contain an identical 23 bp sequence.
Cell
, 16
(4)
, 815-825.
https://doi.org/10.1016/0092-8674(79)90097-7
Identifiers
- DOI
- 10.1016/0092-8674(79)90097-7
- PMID
- 455451