Abstract
Biochemical and electron microscopic analyses of heat-shocked suspension cultures of Peruvian tomato (Lycopersicon peruvianum) revealed that a considerable part of the dominant small heat shock proteins (hsps) with an Mr of approximately 17,000 are structural proteins of newly forming granular aggregates in the cytoplasm (heat shock granules), whose formation strictly depends on heat shock conditions (37 to 40 degrees C) and the presence or simultaneous synthesis of hsps. However, under certain conditions, e.g., in preinduced cultures maintained at 25 degrees C, hsps also accumulate as soluble proteins without concomitant assembly of heat shock granules. Similar heat shock-induced cytoplasmic aggregates were also observed in other cell cultures and heat-shocked tomato leaves and corn coleoptiles.
Keywords
CytoplasmHeat shock proteinBiologyLycopersiconShock (circulatory)Cell biologyColeoptileHeat shockBiophysicsBiochemistryBotanyGene
Affiliated Institutions
Related Publications
Impairment of starvation-induced and constitutive autophagy in <i>Atg7</i> -deficient mice
Autophagy is a membrane-trafficking mechanism that delivers cytoplasmic constituents into the lysosome/vacuole for bulk protein degradation. This mechanism is involved in the pr...
Sequestration of cytoplasmic enzymes in an autophagic vacuole-lysosomal system induced by injection of leupeptin.
Administration of leupeptin to rats induces the accumulation of numerous autophagic vacuoles in the liver. Furuno et al. (Furuno, K., Ishikawa, T., and Kato, K. (1982) J. Bioche...
Publication Info
- Year
- 1983
- Type
- article
- Volume
- 3
- Issue
- 9
- Pages
- 1648-1655
- Citations
- 172
- Access
- Closed
External Links
Social Impact
Altmetric
PlumX Metrics
Social media, news, blog, policy document mentions
Citation Metrics
172
OpenAlex
Cite This
Lutz Nover,
Klaus‐Dieter Scharf,
D. Neumann
(1983).
Formation of cytoplasmic heat shock granules in tomato cell cultures and leaves..
Molecular and Cellular Biology
, 3
(9)
, 1648-1655.
https://doi.org/10.1128/mcb.3.9.1648
Identifiers
- DOI
- 10.1128/mcb.3.9.1648