Abstract
Fluorescent oligonucleotide probes complementary to variable regions of Porphyromonas gingivalis and Bacteroides forsythus 16S ribosomal RNA were used to identify these organisms in smears of formaldehyde-fixed subgingival plaque samples from patients suffering from periodontitis. Fluorescence in situ hybridization represents a useful method for assessing the microbial ecology of the periodontal flora.
Keywords
BiologyBacteroidesPorphyromonas gingivalisMicrobiologyBacteroidaceaePeriodontitis16S ribosomal RNADental plaqueOligomer restrictionFluorescence in situ hybridizationOral microbiologyRibosomal RNAActinomycesFlora (microbiology)BacteriaOligonucleotideDentistryMedicineGeneticsDNA
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Publication Info
- Year
- 1993
- Type
- article
- Volume
- 6
- Issue
- 2-3
- Pages
- 109-114
- Citations
- 22
- Access
- Closed
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Cite This
H Gersdorf,
K. Pelz,
U.B. Gà ̄¿ ⁄ bel
(1993).
Fluorescence in situ hybridization for direct visualization of Gram-negative anaerobes in subgingival plaque samples.
FEMS Immunology & Medical Microbiology
, 6
(2-3)
, 109-114.
https://doi.org/10.1111/j.1574-695x.1993.tb00312.x
Identifiers
- DOI
- 10.1111/j.1574-695x.1993.tb00312.x