Field-deployable viral diagnostics using CRISPR-Cas13

Abstract

Taking CRISPR technology further CRISPR techniques are allowing the development of technologies for nucleic acid detection (see the Perspective by Chertow). Taking advantages of the distinctive enzymatic properties of CRISPR enzymes, Gootenberg et al. developed an improved nucleic acid detection technology for multiplexed quantitative and highly sensitive detection, combined with lateral flow for visual readout. Myhrvold et al. added a sample preparation protocol to create a field-deployable viral diagnostic platform for rapid detection of specific strains of pathogens in clinical samples. Cas12a (also known as Cpf1), a type V CRISPR protein, cleaves double-stranded DNA and has been adapted for genome editing. Chen et al. discovered that Cas12a also processes single-stranded DNA threading activity. A technology platform based on this activity detected human papillomavirus in patient samples with high sensitivity. Science , this issue p. 439 , p. 444 , p. 436 ; see also p. 381

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Affiliated Institutions

• Center for Systems Biology US
• Harvard University US
• Harvard–MIT Division of Health Sciences and Technology US
• National Autonomous University of Honduras HN
• Massachusetts Institute of Technology US
• Faculdade de Medicina de São José do Rio Preto BR
• McGovern Institute for Brain Research US
• Universidade Estadual Paulista (Unesp) BR
• IIT@MIT US
• Broad Institute US
• Howard Hughes Medical Institute US
• Icahn School of Medicine at Mount Sinai US
• Florida Gulf Coast University US

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