Abstract
As a result of advancements in high-throughput technology, the sequencing of the pioneering 16S rRNA gene marker is gradually shedding light on the taxonomic characterization of the spectacular microbial diversity that inhabits the earth.16S rRNA-based investigations of microbial environmental niches are currently conducted using several technologies, including large-scale clonal Sanger sequencing, oligonucleotide microarrays, and, particularly, 454 pyrosequencing that targets specific regions or is linked to barcoding strategies.Interestingly, the short read length produced by next-generation sequencing technology has led to new computational efforts in the taxonomic sequence assignment process.From a medical perspective, the characterization of the microbial composition of the skin surface, oral cavity, and gut in both healthy and diseased people enables a comparison of microbial community profiles and also contributes to the understanding of the potential impact of a particular microbial community.
Keywords
PyrosequencingBiologyComputational biologySanger sequencing16S ribosomal RNADNA sequencingMetagenomicsRibosomal RNAMicrobial population biologyEvolutionary biologyGeneticsGeneBacteria
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Publication Info
- Year
- 2009
- Type
- article
- Volume
- 02
- Issue
- 01
- Citations
- 116
- Access
- Closed
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Cite This
Fabrice Armougom
(2009).
Exploring Microbial Diversity Using 16S rRNA High-Throughput Methods.
Journal of Computer Science & Systems Biology
, 02
(01)
.
https://doi.org/10.4172/jcsb.1000019
Identifiers
- DOI
- 10.4172/jcsb.1000019