Abstract

To investigate the phenotype associated with estrogen receptor alpha (ER) expression in breast carcinoma, gene expression profiles of 58 node-negative breast carcinomas discordant for ER status were determined using DNA microarray technology. Using artificial neural networks as well as standard hierarchical clustering techniques, the tumors could be classified according to ER status, and a list of genes which discriminate tumors according to ER status was generated. The artificial neural networks could accurately predict ER status even when excluding top discriminator genes, including ER itself. By reference to the serial analysis of gene expression database, we found that only a small proportion of the 100 most important ER discriminator genes were also regulated by estradiol in MCF-7 cells. The results provide evidence that ER+ and ER- tumors display remarkably different gene-expression phenotypes not solely explained by differences in estrogen responsiveness.

Keywords

Estrogen receptorEstrogen receptor alphaBreast cancerPhenotypeBiologyGeneEstrogenCancer researchEstrogen receptor betaGene expressionCancerInternal medicineMedicineEndocrinologyGenetics

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Publication Info

Year
2001
Type
article
Volume
61
Issue
16
Pages
5979-84
Citations
682
Access
Closed

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Sofia Gruvberger, Markus Ringnér, Yidong Chen et al. (2001). Estrogen receptor status in breast cancer is associated with remarkably distinct gene expression patterns.. PubMed , 61 (16) , 5979-84.