Abstract
An estimation of the DNA sequence divergence between defined DNA secquences of individuals or species may be made from comparison by gel electrophoresis of restriction endonuclease digests. This analysis is applicable to purified DNA sequence of moderate complexity (1-100 X 10(6) daltons) which have diverged by base substitution of 0.5 to 25% of nucleotides.
Keywords
BiologyRestriction enzymeDNAEndonucleaseDivergence (linguistics)GeneticsSequence (biology)Nucleic acid sequenceDNA sequencingBase pairMolecular biologyRestriction fragmentNucleotideGel electrophoresisSticky and blunt endsGene
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Publication Info
- Year
- 1977
- Type
- article
- Volume
- 4
- Issue
- 5
- Pages
- 1257-1266
- Citations
- 341
- Access
- Closed
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Cite This
William B. Upholt
(1977).
Estimation of DNA sequence divergence from comparison of restriction endonuclease digests.
Nucleic Acids Research
, 4
(5)
, 1257-1266.
https://doi.org/10.1093/nar/4.5.1257
Identifiers
- DOI
- 10.1093/nar/4.5.1257