Abstract
Abstract Many (but not all) of the bioluminescent systems in coelenter‐ates involve energy transfer from an excited product molecule of the calcium activated photoprotein to a second species, the green fluorescent protein, with emission at 508 nm from its excited state. Although all the luminescent coelen‐terates studied possess photoproteins, not all of them have the green fluorescent protein. This green fluorescent molecule is localized in the luminescent cells; they can thus be easily distinguished by fluorescence microscopy. The active components occur in subcellular particles; these have been isolated in an active form by homogenization in isotonic (to sea water) salt solutions.
Keywords
Affiliated Institutions
Related Publications
Wavelength mutations and posttranslational autoxidation of green fluorescent protein.
The green fluorescent protein (GFP) of the jellyfish Aequorea victoria is an unusual protein with strong visible absorbance and fluorescence from a p-hydroxybenzylidene-imidazol...
Chemical structure of the hexapeptide chromophore of the Aequorea green-fluorescent protein
The green-fluorescent proteins (GFP) are a unique class of proteins involved in bioluminescence of many cnidaria. The GFPs serve as energy-transfer acceptors, receiving energy f...
Reducing the Environmental Sensitivity of Yellow Fluorescent Protein
Yellow mutants of the green fluorescent protein (YFP) are crucial constituents of genetically encoded indicators of signal transduction and fusions to monitor protein-protein in...
Far-red fluorescent tag for protein labelling
Practical applications of green fluorescent protein ('GFP')-like fluorescent proteins (FPs) from species of the class Anthozoa (sea anemones, corals and sea pens) are strongly r...
A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells
We report a photoactivatable variant of the Aequorea victoria green fluorescent protein (GFP) that, after intense irradiation with 413-nanometer light, increases fluorescence 10...
Publication Info
- Year
- 1971
- Type
- article
- Volume
- 77
- Issue
- 3
- Pages
- 313-318
- Citations
- 389
- Access
- Closed
External Links
Social Impact
Social media, news, blog, policy document mentions
Citation Metrics
Cite This
Identifiers
- DOI
- 10.1002/jcp.1040770305