eggNOG-mapper v2: Functional Annotation, Orthology Assignments, and Domain Prediction at the Metagenomic Scale

Abstract

Abstract Even though automated functional annotation of genes represents a fundamental step in most genomic and metagenomic workflows, it remains challenging at large scales. Here, we describe a major upgrade to eggNOG-mapper, a tool for functional annotation based on precomputed orthology assignments, now optimized for vast (meta)genomic data sets. Improvements in version 2 include a full update of both the genomes and functional databases to those from eggNOG v5, as well as several efficiency enhancements and new features. Most notably, eggNOG-mapper v2 now allows for: 1) de novo gene prediction from raw contigs, 2) built-in pairwise orthology prediction, 3) fast protein domain discovery, and 4) automated GFF decoration. eggNOG-mapper v2 is available as a standalone tool or as an online service at http://eggnog-mapper.embl.de.

Keywords

Affiliated Institutions

• Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria ES
• University of Würzburg DE
• Yonsei University KR
• European Molecular Biology Laboratory DE
• Centre for Plant Biotechnology and Genomics ES
• Biobyte Solutions (Germany) DE
• Universidad Politécnica de Madrid ES

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