Abstract
"T-Sapphire" and its circular permutations can be used as labels of proteins or cellular structures and as FRET donors in combination with red-fluorescent acceptor proteins such as DsRed, making it possible to completely separate donor and acceptor excitation and emission in intensity-based FRET experiments.
Keywords
Green fluorescent proteinFörster resonance energy transferMutantBiologyFusion proteinMutagenesisFluorescenceBiophysicsFolding (DSP implementation)OrganelleYellow fluorescent proteinGeneCell biologyGeneticsRecombinant DNA
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Publication Info
- Year
- 2003
- Type
- article
- Volume
- 3
- Issue
- 1
- Pages
- 5-5
- Citations
- 173
- Access
- Closed
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Cite This
Otilia Zapata-Hommer,
Oliver Griesbeck
(2003).
Efficiently folding and circularly permuted variants of the Sapphire mutant of GFP.
BMC Biotechnology
, 3
(1)
, 5-5.
https://doi.org/10.1186/1472-6750-3-5
Identifiers
- DOI
- 10.1186/1472-6750-3-5