Abstract

Using fluorescence in situ hybridization we show striking differences in nuclear position, chromosome morphology, and interactions with nuclear substructure for human chromosomes 18 and 19. Human chromosome 19 is shown to adopt a more internal position in the nucleus than chromosome 18 and to be more extensively associated with the nuclear matrix. The more peripheral localization of chromosome 18 is established early in the cell cycle and is maintained thereafter. We show that the preferential localization of chromosomes 18 and 19 in the nucleus is reflected in the orientation of translocation chromosomes in the nucleus. Lastly, we show that the inhibition of transcription can have gross, but reversible, effects on chromosome architecture. Our data demonstrate that the distribution of genomic sequences between chromosomes has implications for nuclear structure and we discuss our findings in relation to a model of the human nucleus that is functionally compartmentalized.

Keywords

NucleusChromosomeCell nucleusBiologyNuclear matrixFluorescence in situ hybridizationChromosomal translocationGeneticsCell biologyGeneChromatin

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Publication Info

Year
1999
Type
article
Volume
145
Issue
6
Pages
1119-1131
Citations
923
Access
Closed

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Cite This

J. A. Croft, Joanna M. Bridger, Shelagh Boyle et al. (1999). Differences in the Localization and Morphology of Chromosomes in the Human Nucleus. The Journal of Cell Biology , 145 (6) , 1119-1131. https://doi.org/10.1083/jcb.145.6.1119

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DOI
10.1083/jcb.145.6.1119