Abstract

Two sets of consensus PCR primers consisting of a common 3' primer CP-I and two 5'-primers, CP-IIG (primer set A) and CP-IIS (primer set B), in the E1 open reading frame of the human papillomavirus (HPV) genome are presented. These two primer sets enabled the detection of a 188 base pair (bp) fragment of HPV 1, 2, 3, 4, 5, 6b, 7, 8, 9, 10a, 11, 12, 14a, 16, 17, 18, 19, 20, 21, 22, 24, 25, 31, 33, 36, 37, 38, 39 and 46. HPV types 15, 23, 49 and 50 were poorly amplified and HPV type 41 was not amplified. The method is suitable for the detection of HPV DNA sequences in clinical samples of both cervical and cutaneous lesions.

Keywords

Primer (cosmetics)BiologyVirologyPolymerase chain reactionGenomeHuman papillomavirusMolecular biologyPapillomaviridaeBase pairDNAGeneticsCervical cancerGeneCancerMedicineInternal medicine

MeSH Terms

Base SequenceCervix UteriConsensus SequenceDNA ProbesDNAViralFemaleHumansMolecular Sequence DataPapillomaviridaePolymerase Chain ReactionSensitivity and SpecificitySequence HomologyNucleic AcidWarts

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Publication Info

Year
1993
Type
article
Volume
42
Issue
2-3
Pages
265-279
Citations
180
Access
Closed

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Cite This

L. M. Tieben, Jan ter Schegget, R. P. Minnaar et al. (1993). Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers. Journal of Virological Methods , 42 (2-3) , 265-279. https://doi.org/10.1016/0166-0934(93)90038-s

Identifiers

DOI
10.1016/0166-0934(93)90038-s
PMID
8390474

Data Quality

Data completeness: 86%