Abstract

ABSTRACT We report the design and evaluation of PCR primers 63f and 1387r for amplification of 16S rRNA genes from bacteria. Their specificity and efficacy were tested systematically with a variety of bacterial species and environmental samples. They were found to be more useful for 16S rRNA gene amplification in ecological and systematic studies than PCR amplimers that are currently more generally used.

Keywords

Biology16S ribosomal RNAGeneBacteriaGeneticsPolymerase chain reactionComputational biologyRibosomal RNABacterial geneticsMicrobiologyEscherichia coli

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Publication Info

Year
1998
Type
article
Volume
64
Issue
2
Pages
795-799
Citations
1796
Access
Closed

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Julian R. Marchesi, Takuichi Sato, Andrew J. Weightman et al. (1998). Design and Evaluation of Useful Bacterium-Specific PCR Primers That Amplify Genes Coding for Bacterial 16S rRNA. Applied and Environmental Microbiology , 64 (2) , 795-799. https://doi.org/10.1128/aem.64.2.795-799.1998

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DOI
10.1128/aem.64.2.795-799.1998