CRISPR–Cas12-based detection of SARS-CoV-2

James P. Broughton , Xianding Deng , Guixia Yu , James P. Broughton , Xianding Deng , Guixia Yu , Clare L. Fasching , Venice Servellita , Jasmeet Singh , Xin Miao , Jessica Streithorst , Andrea Granados , Alicia Sotomayor-González , Kelsey C. Zorn , Allan Gopez , Elaine Hsu , Wei Gu , Steve Miller , Chao‐Yang Pan , Hugo Guevara , Debra A. Wadford , Janice S. Chen , Charles Y. Chiu
2020 Nature Biotechnology 2,614 citations

Abstract

An outbreak of betacoronavirus severe acute respiratory syndrome (SARS)-CoV-2 began in Wuhan, China in December 2019. COVID-19, the disease associated with SARS-CoV-2 infection, rapidly spread to produce a global pandemic. We report development of a rapid (<40 min), easy-to-implement and accurate CRISPR-Cas12-based lateral flow assay for detection of SARS-CoV-2 from respiratory swab RNA extracts. We validated our method using contrived reference samples and clinical samples from patients in the United States, including 36 patients with COVID-19 infection and 42 patients with other viral respiratory infections. Our CRISPR-based DETECTR assay provides a visual and faster alternative to the US Centers for Disease Control and Prevention SARS-CoV-2 real-time RT-PCR assay, with 95% positive predictive agreement and 100% negative predictive agreement.

Keywords

OutbreakSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)VirologyCoronavirus disease 2019 (COVID-19)CRISPRBetacoronavirusPandemic2019-20 coronavirus outbreakMedicineInfection controlBiologyDiseaseInfectious disease (medical specialty)Intensive care medicineInternal medicineGeneGenetics

MeSH Terms

BetacoronavirusCOVID-19COVID-19 TestingCOVID-19 VaccinesCRISPR-Cas SystemsClinical Laboratory TechniquesCoronavirus InfectionsHumansNucleic Acid Amplification TechniquesPandemicsPneumoniaViralRNAGuideCRISPR-Cas SystemsSARS-CoV-2Time Factors

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Publication Info

Year
2020
Type
article
Volume
38
Issue
7
Pages
870-874
Citations
2614
Access
Closed

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Cite This

James P. Broughton, Xianding Deng, Guixia Yu et al. (2020). CRISPR–Cas12-based detection of SARS-CoV-2. Nature Biotechnology , 38 (7) , 870-874. https://doi.org/10.1038/s41587-020-0513-4

Identifiers

DOI
10.1038/s41587-020-0513-4
PMID
32300245
PMCID
PMC9107629

Data Quality

Data completeness: 90%