ConservedPrimers 2.0: A high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP...

Abstract

The ConservedPrimers 2.0 pipeline for designing intron-flanking primers was developed and its utility demonstrated. The tool can be used for SNP discovery, genetic variation assays and marker development for any target genome that has abundant ESTs and a related reference genome that has been fully sequenced. The ConservedPrimers 2.0 pipeline has been implemented as a command-line tool as well as a web application. Both versions are freely available at http://wheat.pw.usda.gov/demos/ConservedPrimers/.

Keywords

Primer (cosmetics)Computational biologyBiologyGeneticsSNPPipeline (software)DNA microarrayIntronGenomeGeneSingle-nucleotide polymorphismComputer scienceGene expressionGenotype

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Publication Info

Year: 2009
Type: article
Volume: 10
Issue: 1
Pages: 331-331
Citations: 37
Access: Closed

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APA Style

                            
                                
                                    Frank M. You, 
                                
                                    Naxin Huo, 
                                
                                    Yong Gu
                                
                                et al.
                            
                            (2009). 
                            ConservedPrimers 2.0: A high-throughput pipeline for comparative genome referenced intron-flanking PCR primer design and its application in wheat SNP discovery. 
                            BMC Bioinformatics
                            , 10
                            (1)
                            , 331-331.
                            https://doi.org/10.1186/1471-2105-10-331
                        

Identifiers

DOI: 10.1186/1471-2105-10-331