Abstract
The primary structures of the 16S rRNAs of Bacillus anthracis, Bacillus cereus, Bacillus mycoides, and Bacillus thuringiensis were determined by using the reverse transcription-dideoxy sequencing method. All of the strains exhibited very high levels of sequence similarity (greater than 99%) that were consistent with the close relationships shown by previous DNA hybridization studies. The sequences of B. anthracis Sterne and B. cereus emetic strain NCTC 11143 were found to be identical for a continuous stretch of 1,446 bases and differed from the sequence of B. cereus NCDO 1771T (T = type strain) by only a single nucleotide. The 16S rRNA sequences of B. mycoides and B. thuringiensis differed from each other and from the sequences of B. anthracis and B. cereus by four to nine nucleotides.
Keywords
Bacillus anthracisBacillus cereusCereusBacillus thuringiensisBiologyMicrobiologyNucleic acid sequence16S ribosomal RNABacillaceaeNucleotideStrain (injury)Reverse transcriptaseBacillus (shape)Sequence analysisGeneticsDNABacteriaGenePolymerase chain reaction
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Publication Info
- Year
- 1991
- Type
- article
- Volume
- 41
- Issue
- 3
- Pages
- 343-346
- Citations
- 473
- Access
- Closed
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Cite This
C. Ash,
J. A. E. FARROW,
M. Dorsch
et al.
(1991).
Comparative Analysis of Bacillus anthracis, Bacillus cereus, and Related Species on the Basis of Reverse Transcriptase Sequencing of 16S rRNA.
International Journal of Systematic Bacteriology
, 41
(3)
, 343-346.
https://doi.org/10.1099/00207713-41-3-343
Identifiers
- DOI
- 10.1099/00207713-41-3-343