Cleavage of Scarecrow-like mRNA Targets Directed by a Class of Arabidopsis miRNA

César Llave; Zhixin Xie; Kristin D. Kasschau; James C. Carrington

doi:10.1126/science.1076311

Abstract

Micro-RNAs (miRNAs) are regulatory molecules that mediate effects by interacting with messenger RNA (mRNA) targets. Here we show that Arabidopsis thaliana miRNA 39 (also known as miR171), a 21-ribonucleotide species that accumulates predominantly in inflorescence tissues, is produced from an intergenic region in chromosome III and functionally interacts with mRNA targets encoding several members of the Scarecrow-like ( SCL ) family of putative transcription factors. miRNA 39 is complementary to an internal region of three SCL mRNAs. The interaction results in specific cleavage of target mRNA within the region of complementarity, indicating that this class of miRNA functions like small interfering RNA associated with RNA silencing to guide sequence-specific cleavage in a developmentally controlled manner.

Keywords

BiologymicroRNAMessenger RNAGene silencingRNAGeneticsArabidopsisCleavage (geology)RNA silencingNon-coding RNACell biologyTranscription (linguistics)Molecular biologyRNA interferenceGene

Affiliated Institutions

Oregon State University US

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Publication Info

Year: 2002
Type: article
Volume: 297
Issue: 5589
Pages: 2053-2056
Citations: 1600
Access: Closed

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APA Style

                            
                                
                                    César Llave, 
                                
                                    Zhixin Xie, 
                                
                                    Kristin D. Kasschau
                                
                                et al.
                            
                            (2002). 
                            Cleavage of <i>Scarecrow-like</i> mRNA Targets Directed by a Class of <i>Arabidopsis</i> miRNA. 
                            Science
                            , 297
                            (5589)
                            , 2053-2056.
                            https://doi.org/10.1126/science.1076311
                        

Identifiers

DOI: 10.1126/science.1076311